5M8G
Tubulin-MTD265 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-08 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.768, 157.508, 179.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.292 - 2.147 |
R-factor | 0.1729 |
Rwork | 0.171 |
R-free | 0.20770 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4i4t |
RMSD bond length | 0.007 |
RMSD bond angle | 0.893 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.292 | 2.210 |
High resolution limit [Å] | 2.147 | 2.150 |
Rmerge | 0.128 | 2.573 |
Number of reflections | 161400 | |
<I/σ(I)> | 17.6 | 1.1 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 13.5 | 12.8 |
CC(1/2) | 0.999 | 0.414 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.7 | 293 | 3% PEG 4000, 4-6% GLYCEROL, 30 MM MAGNESIUM CHLORIDE, 30 MM CALCIUM CHLORIDE, 100 MM MES/IMIDAZOLE |