5M8E
Crystal structure of a GH43 arabonofuranosidase from Weissella sp. strain 142
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0384 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.880, 71.930, 79.510 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 29.700 - 2.000 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.21600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3akh |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.700 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.069 | 0.395 |
| <I/σ(I)> | 13.1 | 3.4 |
| Completeness [%] | 98.2 | 92.4 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 27 to 29 % PEG 1500, 60 to 140 mM malonate-imidazole-borate (MIB) buffer, pH 4.0 to 4.5 |
