5M8C
Spliceosome component
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-10-10 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.220, 100.140, 65.340 |
Unit cell angles | 90.00, 92.33, 90.00 |
Refinement procedure
Resolution | 48.992 - 2.300 |
R-factor | 0.1805 |
Rwork | 0.177 |
R-free | 0.23480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lrv |
RMSD bond length | 0.008 |
RMSD bond angle | 0.876 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.992 | 48.992 | 2.400 |
High resolution limit [Å] | 2.300 | 10.000 | 2.300 |
Rmerge | 0.069 | 0.023 | 0.376 |
Rmeas | 0.082 | 0.027 | 0.448 |
Total number of observations | 107782 | ||
Number of reflections | 31956 | 407 | 3784 |
<I/σ(I)> | 12.35 | 40.1 | 3.04 |
Completeness [%] | 99.3 | 97.8 | 99 |
Redundancy | 3.373 | 3.462 | 3.357 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M MOPS pH 7.0 12% PEG 4000 |