5M8B
Crystal structure of alpha-L-arabinofuranosidase from Lactobacillus brevis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I911-3 |
Synchrotron site | MAX II |
Beamline | I911-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 132.148, 132.148, 266.925 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.380 - 1.900 |
R-factor | 0.147 |
Rwork | 0.146 |
R-free | 0.17400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ARABINOFURANOSIDASE FROM WEISSELLA SP142 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.108 | 0.836 |
Number of reflections | 70624 | |
<I/σ(I)> | 16.2 | 2.4 |
Completeness [%] | 99.9 | 98.8 |
Redundancy | 7.3 | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 20% PEG 3350 and 0.2M sodium iodide |