5M8B
Crystal structure of alpha-L-arabinofuranosidase from Lactobacillus brevis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 132.148, 132.148, 266.925 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.380 - 1.900 |
| R-factor | 0.147 |
| Rwork | 0.146 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ARABINOFURANOSIDASE FROM WEISSELLA SP142 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.108 | 0.836 |
| Number of reflections | 70624 | |
| <I/σ(I)> | 16.2 | 2.4 |
| Completeness [%] | 99.9 | 98.8 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 20% PEG 3350 and 0.2M sodium iodide |
