5M89
Spliceosome component
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.229, 103.715, 74.389 |
| Unit cell angles | 90.00, 101.77, 90.00 |
Refinement procedure
| Resolution | 43.299 - 1.605 |
| R-factor | 0.1662 |
| Rwork | 0.165 |
| R-free | 0.18570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5m8c |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.823 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.061 | |
| Number of reflections | 176701 | |
| <I/σ(I)> | 15.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 293 | 0.1 M Citric Acid Anhydrous pH 4.0 20% PEG 6000 |
