5M83
Translation initiation factor 4E in complex with (RP)-m2(7,2'O)GppSpA mRNA 5' cap analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 47.060, 59.740, 149.180 |
Unit cell angles | 90.00, 96.32, 90.00 |
Refinement procedure
Resolution | 37.068 - 1.860 |
R-factor | 0.207 |
Rwork | 0.206 |
R-free | 0.25140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l8b |
RMSD bond length | 0.007 |
RMSD bond angle | 0.969 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.910 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 0.070 | 0.909 |
Number of reflections | 34747 | |
<I/σ(I)> | 16.57 | 2.02 |
Completeness [%] | 99.7 | 98.1 |
Redundancy | 6.436 | 6.45 |
CC(1/2) | 0.999 | 0.787 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 16% PEG 10000, 0.2M CH3COONa, 0.1M TRIS pH 8.0 |