5M7X

Translation initiation factor 4E in complex with (RP)-m2(7,2'O)GppSepG mRNA 5' cap analog (beta-Se-ARCA D1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97957
Spacegroup name	P 1
Unit cell lengths	38.110, 38.160, 147.070
Unit cell angles	88.55, 95.32, 103.33

Refinement procedure

Resolution	48.812 - 1.680
R-factor	0.1924
Rwork	0.192
R-free	0.21880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l8b
RMSD bond length	0.006
RMSD bond angle	1.039
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.720
High resolution limit [Å]	1.680	1.680
Rmerge	0.039	0.351
Number of reflections	162380
<I/σ(I)>	11.15	1.97
Completeness [%]	88.4	81.9
Redundancy	1.606	1.543
CC(1/2)	0.998	0.742

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	20% PEG 5000 MME, 0.2M HCOONa, 0.1M BICINE pH 8.5