5M7D
Blood group synthase AAGlyB in complex with UDP-Gal and cryoprotected with glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-08-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0723 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 52.643, 148.796, 79.782 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.200 |
R-factor | 0.13163 |
Rwork | 0.130 |
R-free | 0.15654 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.013 |
RMSD bond angle | 1.671 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.140 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.088 | 0.522 |
Number of reflections | 96982 | |
<I/σ(I)> | 10.4 | 2.1 |
Completeness [%] | 99.1 | 99.3 |
Redundancy | 3.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MOPS buffer, manganese chloride, ammonium sulfate, PEG 3350 |