5M6Q
Crystal Structure of Kutzneria albida transglutaminase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-20 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 3 |
Unit cell lengths | 106.870, 106.870, 56.080 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.276 - 1.980 |
R-factor | 0.1849 |
Rwork | 0.183 |
R-free | 0.22960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iu0 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.7) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.960 | 2.030 | |
High resolution limit [Å] | 1.980 | 8.860 | 1.980 |
Rmerge | 0.179 | 0.040 | 1.529 |
Number of reflections | 49849 | ||
<I/σ(I)> | 7.41 | 27.72 | 1.06 |
Completeness [%] | 99.9 | 99.5 | 99.8 |
Redundancy | 4.91 | 4.6 | |
CC(1/2) | 0.993 | 0.997 | 0.301 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2 M ammonium tartrate, 20% PEG 3350 |