5M6J
Crystal structure of nitrophorin 7 E27V mutant from Rhodnius prolixus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-07-26 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.91841 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 38.228, 66.885, 38.714 |
Unit cell angles | 90.00, 116.57, 90.00 |
Refinement procedure
Resolution | 34.626 - 1.700 |
R-factor | 0.1635 |
Rwork | 0.161 |
R-free | 0.21260 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xmc |
RMSD bond length | 0.006 |
RMSD bond angle | 1.057 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX (2011_08_24_1020) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.630 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.057 | 0.488 |
Number of reflections | 72215 | |
<I/σ(I)> | 17.6 | 3 |
Completeness [%] | 96.6 | 95.3 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | NaOAc/HOAc |