5M6G

Crystal structure Glucan 1,4-beta-glucosidase from Saccharopolyspora erythraea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.033121
Spacegroup name	P 21 21 21
Unit cell lengths	64.292, 75.717, 113.893
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.511 - 1.829
R-factor	0.173
Rwork	0.172
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ieq
RMSD bond length	0.006
RMSD bond angle	0.819
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.829
Number of reflections	47457
<I/σ(I)>	11.27
Completeness [%]	95.1
Redundancy	3.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	15% PEG 20K, 100mM MES Sodium Salt, 80 mM Mangan II Chloride