5M5V
Clathrin heavy chain N-terminal domain bound to a clathrin-box motif from hepatitis D virus large antigen (clade 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-07 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 136.900, 131.230, 78.450 |
Unit cell angles | 90.00, 115.86, 90.00 |
Refinement procedure
Resolution | 39.200 - 1.960 |
R-factor | 0.17037 |
Rwork | 0.169 |
R-free | 0.19343 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c9i |
RMSD bond length | 0.019 |
RMSD bond angle | 1.917 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 2.000 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.081 | 0.581 |
Number of reflections | 79976 | |
<I/σ(I)> | 7.2 | 1.5 |
Completeness [%] | 94.6 | 95.9 |
Redundancy | 2.5 | 2.3 |
CC(1/2) | 0.996 | 0.563 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 200 nL protein:peptide (20 mg/mL NTD and 3.6 mM peptide) plus 400 nL reservoir equilibrated against a 80 uL reservoir of 1.75 M sodium malonate pH 7.0 |