5M5S
Clathrin heavy chain N-terminal domain bound to amphiphysin clathrin-box motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.92819 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 139.967, 134.110, 78.012 |
| Unit cell angles | 90.00, 115.07, 90.00 |
Refinement procedure
| Resolution | 67.060 - 1.880 |
| R-factor | 0.20565 |
| Rwork | 0.204 |
| R-free | 0.23388 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9i |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.396 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.100 | 1.930 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.149 | 1.092 |
| Number of reflections | 99868 | |
| <I/σ(I)> | 6.5 | 1.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.1 | 4.4 |
| CC(1/2) | 0.993 | 0.568 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1 uL protein:peptide mix (14 mg/mL NTD and 3.4 mM peptide) plus 2 uL reservoir equilibrated against a 200 uL reservoir of 0.85 M sodium malonate pH 7.5 |
