5M5R
Clathrin heavy chain N-terminal domain bound to beta2 adaptin clathrin box motif
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92818 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 108.144, 133.177, 77.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.100 - 1.760 |
R-factor | 0.17725 |
Rwork | 0.176 |
R-free | 0.20516 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c9i |
RMSD bond length | 0.016 |
RMSD bond angle | 1.719 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.100 | 1.810 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.053 | 1.538 |
Number of reflections | 52951 | |
<I/σ(I)> | 15.8 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.5 | 7 |
CC(1/2) | 1.000 | 0.672 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 293 | 1 uL protein plus peptide mix (14 mg/mL NTD and 3.4 mM peptide) plus 2 uL reservoir equilibrated against a 200 uL reservoir of 0.94 M sodium malonate pH 6.7 |