5M5M

Pseudo-atomic model of microtubule-bound S.pombe kinesin-5 motor domain in the AMPPNP state (based on cryo-electron microscopy experiment): the N-terminus adopts multiple conformations.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK I

EM Data Collection

Microscope	FEI TECNAI F20
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Detector	GATAN ULTRASCAN 4000 (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	9.3
Software	EMAN,SPIDER,FREALIGN,Flex-EM,MODELLER,CTFFIND,UCSF Chimera
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION