5M48
Coiled coil domain of Rtt103p
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979, 0.9792, 0.9919, 0.9713 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 217.141, 217.141, 217.141 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.554 - 2.593 |
R-factor | 0.2474 |
Rwork | 0.245 |
R-free | 0.26400 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.463 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.9-1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.540 | 2.681 |
High resolution limit [Å] | 2.593 | 2.589 |
Number of reflections | 14200 | |
<I/σ(I)> | 21.9 | |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 29.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Purified protein was dialysed to 25mM Tris 200mM NaCl 1mM BME, pH = 8.0 (4oC) and concentrated to 6mg/ml. Protein was crystallized in 3.75M sodium formate. |