5M3J
Influenza B polymerase bound to four heptad repeats of serine 5 phosphorylated Pol II CTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 200.160, 200.160, 250.380 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.070 - 3.500 |
R-factor | 0.2401 |
Rwork | 0.239 |
R-free | 0.26820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wsa |
RMSD bond length | 0.002 |
RMSD bond angle | 0.472 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.600 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.191 | 2.220 |
Number of reflections | 73405 | |
<I/σ(I)> | 8.38 | 1.02 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.54 | 8.63 |
CC(1/2) | 0.998 | 0.547 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 277 | FluB polymerase in a buffer containing 500 mM NaCl, 50 mM Hepes pH 7.5, 5% glycerol and 2mM Tris(2-carboxyethyl)phosphine (TCEP) was mixed with 5' and 3' ends of vRNA promoter and CTD peptide. Reservoir solution contained 0.1 M bicine pH 9.0, 10% MPD. |