5M38
Structure of the TagL peptidoglycan binding domain from EAEC T6SS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-06 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.8729 |
Spacegroup name | I 41 3 2 |
Unit cell lengths | 211.550, 211.550, 211.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.100 - 2.600 |
R-factor | 0.2176 |
Rwork | 0.217 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3td5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.310 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | 2.710 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.088 | |
Number of reflections | 25065 | |
<I/σ(I)> | 18.5 | 1.5 |
Completeness [%] | 99.8 | 99.5 |
Redundancy | 9 | 9.5 |
CC(1/2) | 0.999 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | mixing protein solution (5 mg/ml) with 0.1 M imidazole pH 6.5, 1.2 M NaAc |