5M30
Structure of TssK from T6SS EAEC in complex with nanobody nb18
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.240, 153.670, 154.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.920 - 2.600 |
R-factor | 0.2079 |
Rwork | 0.207 |
R-free | 0.22630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.210 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.660 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.018 | 1.400 |
Number of reflections | 69265 | |
<I/σ(I)> | 19.6 | 0.5 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 11 | 10.7 |
CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Complex at 10 mg per ml mixes to 0.2 MgCl2, 10 % PEK 6000, 0.1 M Tris pH 8.3. |