5M2U
The Structure of the Ycf54 protein from Synechocystis sp. PCC6803
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.520, 91.150, 120.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.760 - 2.200 |
R-factor | 0.20775 |
Rwork | 0.206 |
R-free | 0.24958 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5m2p |
RMSD bond length | 0.012 |
RMSD bond angle | 1.467 |
Data reduction software | xia2 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.800 | 2.260 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.050 | 0.790 |
Rpim | 0.020 | 0.350 |
Number of reflections | 31240 | |
<I/σ(I)> | 22.6 | 2.8 |
Completeness [%] | 99.1 | 98.5 |
Redundancy | 6.6 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 290 | 2.2M ammonium sulphate |