5M2D

CRYSTAL STRUCTURE 4Ac Endoglucanase-like protein from Acremonium chrysogenum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	I 1 2 1
Unit cell lengths	72.889, 49.255, 117.552
Unit cell angles	90.00, 93.03, 90.00

Refinement procedure

Resolution	40.793 - 2.097
R-factor	0.1909
Rwork	0.190
R-free	0.21420
RMSD bond length	0.008
RMSD bond angle	0.853
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.000
High resolution limit [Å]	2.090
Number of reflections	23601
<I/σ(I)>	7.15
Completeness [%]	95.2
Redundancy	3.67

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	295	0.2 M Sodium malonate, 20% PEG 3350