5M1Q
Crystal structure of the large terminase nuclease from thermophilic phage G20c with bound Zinc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-07 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 65 |
| Unit cell lengths | 60.983, 60.983, 90.396 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.600 - 1.450 |
| R-factor | 0.2019 |
| Rwork | 0.201 |
| R-free | 0.21100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | in house model |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.600 | 45.600 | 1.470 |
| High resolution limit [Å] | 1.450 | 7.940 | 1.450 |
| Rmerge | 0.086 | 0.047 | 1.291 |
| Rmeas | 0.097 | ||
| Rpim | 0.045 | ||
| Total number of observations | 151672 | ||
| Number of reflections | 33635 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 99.7 | 98.1 | 99.5 |
| Redundancy | 4.5 | 4.4 | 3.9 |
| CC(1/2) | 0.997 | 0.997 | 0.420 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.01 M Zinc chloride, 0.1 M MES pH 6.0, 20% PEG 6000 |
