5M1O
Crystal structure of the large terminase nuclease from thermophilic phage G20c with bound Cobalt
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.937, 53.895, 69.751 |
Unit cell angles | 90.00, 91.62, 90.00 |
Refinement procedure
Resolution | 42.920 - 1.600 |
R-factor | 0.1868 |
Rwork | 0.185 |
R-free | 0.22400 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.273 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.920 | 42.920 | 1.630 |
High resolution limit [Å] | 1.600 | 8.760 | 1.600 |
Rmerge | 0.072 | 0.028 | 0.725 |
Rmeas | 0.092 | ||
Rpim | 0.057 | ||
Total number of observations | 86303 | ||
Number of reflections | 40243 | ||
<I/σ(I)> | 6.6 | ||
Completeness [%] | 95.7 | 90.1 | 89.5 |
Redundancy | 2.1 | 2.2 | 2 |
CC(1/2) | 0.997 | 0.998 | 0.604 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium Tartrate, 0.1 M Bis-tris pH 5.5-6.5, 20% PEG 3350 |