5M1K
Crystal structure of the large terminase nuclease from thermophilic phage G20c with bound Magnesium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-03-06 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9796 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.807, 54.057, 69.950 |
Unit cell angles | 90.00, 91.94, 90.00 |
Refinement procedure
Resolution | 43.780 - 1.200 |
R-factor | 0.1845 |
Rwork | 0.183 |
R-free | 0.20870 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.285 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.780 | 43.780 | 1.220 |
High resolution limit [Å] | 1.200 | 6.570 | 1.200 |
Rmerge | 0.073 | 0.043 | 0.765 |
Rmeas | 0.091 | ||
Rpim | 0.054 | ||
Total number of observations | 250958 | ||
Number of reflections | 98722 | ||
<I/σ(I)> | 7.4 | ||
Completeness [%] | 97.0 | 94 | 97.6 |
Redundancy | 2.5 | 2.7 | 2.5 |
CC(1/2) | 0.996 | 0.991 | 0.522 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Ammonium Tartrate, 0.1 M Bis-tris pH 5.5-6.5, 20% PEG 3350 |