5M1F
Crystal structure of the large terminase nuclease from thermophilic phage G20c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-21 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 55.102, 55.102, 95.759 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.720 - 2.150 |
| R-factor | 0.2044 |
| Rwork | 0.200 |
| R-free | 0.28110 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.27) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.720 | 47.720 | 2.210 |
| High resolution limit [Å] | 2.150 | 9.110 | 2.150 |
| Rmerge | 0.043 | 0.017 | 0.804 |
| Rmeas | 0.055 | 0.021 | 1.043 |
| Rpim | 0.035 | 0.013 | 0.658 |
| Total number of observations | 20099 | 245 | 1569 |
| Number of reflections | 9183 | ||
| <I/σ(I)> | 10.3 | 31.5 | 1 |
| Completeness [%] | 95.6 | 93 | 90.6 |
| Redundancy | 2.2 | 1.7 | 2.2 |
| CC(1/2) | 0.999 | 1.000 | 0.365 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2 M Lithium Sulfate, 0.1 M Bis-tris pH 5.5, 25% PEG3350 |
