5M1B
Crystal structure of C-terminally tagged apo-UbiD from E. coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-01-28 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.044 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 210.187, 210.187, 109.010 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.900 - 3.150 |
R-factor | 0.22384 |
Rwork | 0.221 |
R-free | 0.27655 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2idb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.626 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.900 | 3.230 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.146 | 0.976 |
Number of reflections | 51401 | |
<I/σ(I)> | 14.1 | 3.3 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.5 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294 | Crystals grown in 0.1 mM SPG pH 8, 25 w/v PEG 1500 Crystals were cryo-protected using 10 PEG 400 in mother liquor followed by rapid cryocooling. |