5M0Y
Crystal Structure of the CohScaA-XDocCipB type II complex from Clostridium thermocellum at 1.5Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-30 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.408, 63.741, 141.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.300 - 1.500 |
| R-factor | 0.19117 |
| Rwork | 0.191 |
| R-free | 0.20323 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bm3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.243 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.600 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.650 |
| Number of reflections | 278906 | |
| <I/σ(I)> | 18.8 | 2.3 |
| Completeness [%] | 99.5 | 99.6 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M AMMONIUM SULPHATE 0.1 M REMARK 280 ACETATE PH4.5 30% PEG 400 |
