5M0W
N-terminal domain of mouse Shisa 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 59.765, 59.765, 48.591 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.390 |
| R-factor | 0.11644 |
| Rwork | 0.115 |
| R-free | 0.13668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Single sulphur |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.713 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.880 | 1.410 |
| High resolution limit [Å] | 1.390 | 1.390 |
| Rmerge | 0.030 | 0.456 |
| Number of reflections | 18352 | |
| <I/σ(I)> | 30.5 | 3.4 |
| Completeness [%] | 99.3 | 88.3 |
| Redundancy | 6.8 | 3.2 |
| CC(1/2) | 1.000 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 2.0 M (NH4)2SO4, 100 mM MES pH 6.5, 4% PEG 400 |
