5M0T
Alpha-ketoglutarate-dependent non-heme iron oxygenase EasH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I02 |
Synchrotron site | Diamond |
Beamline | I02 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-06 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.250, 100.170, 148.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.470 - 2.200 |
R-factor | 0.2191 |
Rwork | 0.218 |
R-free | 0.24430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nao |
RMSD bond length | 0.009 |
RMSD bond angle | 1.378 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.7) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.470 | 47.470 | 2.260 |
High resolution limit [Å] | 2.200 | 9.840 | 2.200 |
Rmerge | 0.151 | 0.035 | 1.413 |
Number of reflections | 31397 | ||
<I/σ(I)> | 8.7 | 1.3 | |
Completeness [%] | 99.7 | 99 | 99.5 |
Redundancy | 5.9 | 4.8 | 5.7 |
CC(1/2) | 0.996 | 0.999 | 0.539 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | NULL |