5LZ6

Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of Aichivirus B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979810
Spacegroup name	P 31 2 1
Unit cell lengths	55.630, 55.630, 144.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.177 - 2.600
R-factor	0.2171
Rwork	0.215
R-free	0.26030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lz1
RMSD bond length	0.003
RMSD bond angle	0.708
Data reduction software	XDS (version Oct 15, 2015)
Data scaling software	XDS (version Oct 15, 2015)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.180	2.693
High resolution limit [Å]	2.600	2.600
Rmerge	0.078	1.984
Number of reflections	8472
<I/σ(I)>	22.47	1.24
Completeness [%]	100.0	100
Redundancy	10.4	10.8
CC(1/2)	1.000	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100mM MOPSO/Bis-Tris pH 6.5, 15% w/v PEG 3.000, 1% w/v DDAO, 10% 1,4-butanediol, 10% w/v glucose, 20mM L-arginine/L-threonine/L-histidine/betaine, 10mM trans-4-hydroxy-L-proline