5LZ3

Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of Aichivirus A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-27
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.541870
Spacegroup name	I 2 2 2
Unit cell lengths	54.100, 81.150, 117.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.360 - 3.000
R-factor	0.2199
Rwork	0.218
R-free	0.24810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5lz1
RMSD bond length	0.005
RMSD bond angle	0.768
Data reduction software	XDS (version Oct 15, 2015)
Data scaling software	XDS (version Oct 15, 2015)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.360	3.107
High resolution limit [Å]	3.000	3.000
Rmerge	0.203	0.697
Total number of observations	21415
Number of reflections	5185
<I/σ(I)>	7.9	1.68
Completeness [%]	95.3	96.55
Redundancy	4.1	4.3
CC(1/2)	0.981	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100mM MOPS/HEPES pH 7.5, 12.5% w/v PEG 1.000, 12.5% w/v PEG 3.350, 12.5% v/v MPD, 20mM D-glucose/D-mannose/D-galactose/L-fucose/D-xylose/N-acetyl-D-glucosamine