5LZ3
Crystal structure of human ACBD3 GOLD domain in complex with 3A protein of Aichivirus A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.541870 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 54.100, 81.150, 117.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.360 - 3.000 |
R-factor | 0.2199 |
Rwork | 0.218 |
R-free | 0.24810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lz1 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.768 |
Data reduction software | XDS (version Oct 15, 2015) |
Data scaling software | XDS (version Oct 15, 2015) |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.360 | 3.107 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.203 | 0.697 |
Total number of observations | 21415 | |
Number of reflections | 5185 | |
<I/σ(I)> | 7.9 | 1.68 |
Completeness [%] | 95.3 | 96.55 |
Redundancy | 4.1 | 4.3 |
CC(1/2) | 0.981 | 0.691 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100mM MOPS/HEPES pH 7.5, 12.5% w/v PEG 1.000, 12.5% w/v PEG 3.350, 12.5% v/v MPD, 20mM D-glucose/D-mannose/D-galactose/L-fucose/D-xylose/N-acetyl-D-glucosamine |