5LZ0
Llama nanobody PorM_01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8726 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 78.327, 65.828, 48.314 |
| Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
| Resolution | 27.160 - 1.600 |
| R-factor | 0.205 |
| Rwork | 0.203 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tvs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.800 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 31426 | |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 97.7 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0,1M Tri-Sodium Citrate 20% (w/v) PEG 3000 |
