5LZ0
Llama nanobody PorM_01
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-09-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.8726 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 78.327, 65.828, 48.314 |
Unit cell angles | 90.00, 95.89, 90.00 |
Refinement procedure
Resolution | 27.160 - 1.600 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.23200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4tvs |
RMSD bond length | 0.010 |
RMSD bond angle | 0.970 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.800 | 1.690 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 31426 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 97.7 | |
Redundancy | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0,1M Tri-Sodium Citrate 20% (w/v) PEG 3000 |