5LYW
CRYSTAL STRUCTURE OF HUMAN METHIONINE AMINOPEPTIDASE-2 IN COMPLEX; WITH AN INHIBITOR 6-((R)-2-o-Tolyloxymethyl-pyrrolidin-1-yl)-9H-purine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-06-01 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.780, 100.230, 100.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 16.130 - 1.690 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Refinement software | BUSTER-TNT (2.11.6) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.000 | 1.640 | |
High resolution limit [Å] | 1.600 | 7.160 | 1.600 |
Rmerge | 0.057 | 0.036 | 1.025 |
Number of reflections | 59218 | ||
<I/σ(I)> | 15.7 | 32.88 | 2.22 |
Completeness [%] | 98.9 | 50.1 | 99.9 |
Redundancy | 6.1 | ||
CC(1/2) | 0.999 | 0.997 | 0.768 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 24% methanol, 100 mM citrate |