5LYV

The crystal structure of 7SK 5'-hairpin - Osmium derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.13956
Spacegroup name	P 1 21 1
Unit cell lengths	47.120, 47.740, 68.940
Unit cell angles	90.00, 105.20, 90.00

Refinement procedure

Resolution	40.000 - 2.350
R-factor	0.2001
Rwork	0.199
R-free	0.23170
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lys
RMSD bond length	0.010
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER-TNT (2.10.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.062	0.028	0.458
Rmeas	0.083	0.037	0.644
Total number of observations	41939
Number of reflections	22741	235	1368
<I/σ(I)>	7.37	22.45	1.3
Completeness [%]	93.4	83.9	75.3
Redundancy	1.8
CC(1/2)	0.995	0.996	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	30% PEG 1000, 50 mM Tris pH 7.5, 50 to 75 mM NaCl, 50 mM MgCl2, 3% DMSO