5LYP
Crystal structure of the Tpr Domain of Sgt2.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 298.15 |
Detector technology | PIXEL |
Collection date | 2016-03-03 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 36.861, 50.760, 67.116 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.558 - 1.550 |
R-factor | 0.1948 |
Rwork | 0.194 |
R-free | 0.21850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vyi |
RMSD bond length | 0.005 |
RMSD bond angle | 0.619 |
Data reduction software | xia2 |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 36.860 | 36.860 | 1.590 |
High resolution limit [Å] | 1.550 | 6.930 | 1.550 |
Rmerge | 0.136 | 0.094 | 0.562 |
Number of reflections | 18957 | ||
<I/σ(I)> | 13.9 | ||
Completeness [%] | 100.0 | 99.5 | 100 |
Redundancy | 12.2 | 10.7 | 12.4 |
CC(1/2) | 0.996 | 0.990 | 0.982 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 6 | 293.15 | 0.1 M SPG, pH 6.0, 25 % w/v PEG 1500 |