5LYB
Crystal structure of the S.cerevisiae 80S ribosome in complex with the A-site bound aminoacyl-tRNA analog ACCPmn
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 436.180, 288.240, 303.580 |
Unit cell angles | 90.00, 98.87, 90.00 |
Refinement procedure
Resolution | 172.587 - 3.250 |
R-factor | 0.2086 |
Rwork | 0.208 |
R-free | 0.24880 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.262 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.10.1_2155) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 200.000 | 3.300 | |
High resolution limit [Å] | 3.250 | 150.000 | 3.250 |
Rmerge | 0.422 | 0.015 | 5.801 |
Number of reflections | 1159737 | ||
<I/σ(I)> | 7.87 | 26.61 | 0.99 |
Completeness [%] | 100.0 | 23.1 | 100 |
Redundancy | 5 | ||
CC(1/2) | 0.998 | 1.000 | 0.387 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 277 | Tris-Acetate pH 7.0, KSCN, MgOAc2, glycerol, spermidine, PEG20K |