5LY0
Crystal structure of LOB domain of Ramosa2 from Wheat
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.345, 85.206, 93.334 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.667 - 1.877 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.23070 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.525 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | autoSHARP |
| Refinement software | PHENIX ((dev_2443: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 1.944 |
| High resolution limit [Å] | 1.877 | 1.877 |
| Rmerge | 0.052 | 0.800 |
| Number of reflections | 19838 | |
| <I/σ(I)> | 20.82 | 2.25 |
| Completeness [%] | 99.5 | 99.44 |
| Redundancy | 6.3 | 6.5 |
| CC(1/2) | 0.999 | 0.745 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | Sodium Citrate tribasic dihydrate 0.1M ph 5.5 PEG 1000 22% |
