5LXU

Structure of the DNA-binding domain of LUX ARRHYTHMO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97886
Spacegroup name	P 1 2 1
Unit cell lengths	42.160, 32.830, 53.760
Unit cell angles	90.00, 98.61, 90.00

Refinement procedure

Resolution	41.680 - 2.140
R-factor	0.197
Rwork	0.195
R-free	0.23200
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.910
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.000		2.220
High resolution limit [Å]	2.140	4.610	2.140
Rmerge	0.032	0.019	0.452
Number of reflections	13640
<I/σ(I)>	13.48	32.79	1.59
Completeness [%]	86.7	91.6	54.9
Redundancy	1.47		0.88
CC(1/2)	0.999	0.999	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1M bis-tris propane, 20% PEG 3350, 0.2M sodium malonate