5LXR

Structure of the minimal RBM7 - ZCCHC8 Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.6
Spacegroup name	P 63 2 2
Unit cell lengths	79.519, 79.519, 87.609
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	87.610 - 2.000
Rwork	0.202
R-free	0.22860
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol (1.10.1)
Refinement software	PHENIX (1.10.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	87.610	2.080
High resolution limit [Å]	2.000	2.000
Rmerge	0.187
Number of reflections	11470
<I/σ(I)>	26.6
Completeness [%]	98.8	92.7
Redundancy	134.8
CC(1/2)	1.000	0.341

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291.15	0.1 M Bis-Tris-Propane pH 6.5 0.2 M NaBr 18% (v/v) PEG3350 7% (w/v) glycerol