5LXE

F420-dependent glucose-6-phosphate dehydrogenase from Rhodococcus jostii RHA1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-31
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.71, 2.25
Spacegroup name	P 21 21 21
Unit cell lengths	81.540, 88.120, 88.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.540 - 1.470
R-factor	0.15778
Rwork	0.156
R-free	0.18530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b4y
RMSD bond length	0.021
RMSD bond angle	2.020
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.780	1.500
High resolution limit [Å]	1.470	1.470
Rmerge	0.050	0.550
Number of reflections	106774
<I/σ(I)>	10.9
Completeness [%]	98.7
Redundancy	3.9
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	ammonium sulfate, sodium acetate pH 4.6, PEG 4000