5LX7
Cys-Gly dipeptidase GliJ mutant D38N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-18 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 99.680, 99.680, 107.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 1.950 |
R-factor | 0.16542 |
Rwork | 0.164 |
R-free | 0.19458 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lx0 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.113 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.050 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.043 | 0.563 |
Number of reflections | 45387 | |
<I/σ(I)> | 19.8 | 2.8 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.1 M MES pH 6.0, 5% isopropanol |