Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.063, 123.671, 38.688 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.491 - 2.579 |
| R-factor | 0.2057 |
| Rwork | 0.203 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5LHO |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.524 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.490 | 2.671 |
| High resolution limit [Å] | 2.579 | 2.579 |
| Rmerge | 0.167 | 1.045 |
| Number of reflections | 10043 | |
| <I/σ(I)> | 6.5 | 1.56 |
| Completeness [%] | 98.3 | 98.25 |
| Redundancy | 3.9 | 4.1 |
| CC(1/2) | 0.989 | 0.506 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | MES, PEG 8000, ethylene glycol |
