5LWH
CeuE (Y288F variant) a periplasmic protein from Campylobacter jejuni.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-18 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.521, 65.521, 145.654 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 56.810 - 1.470 |
| R-factor | 0.1301 |
| Rwork | 0.128 |
| R-free | 0.16196 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zkw |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.456 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.810 | 1.500 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Rmerge | 0.045 | 1.267 |
| Number of reflections | 62481 | |
| <I/σ(I)> | 21.7 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.7 | 8.9 |
| CC(1/2) | 1.000 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 0.01M ZnCl2; 0.1M MES, pH 6.0; 20% Peg 6000 |
