5LVX
Crystal structure of glucocerebrosidase with an inhibitory quinazoline modulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.910, 132.281, 103.085 |
| Unit cell angles | 90.00, 95.26, 90.00 |
Refinement procedure
| Resolution | 102.650 - 2.200 |
| R-factor | 0.18907 |
| Rwork | 0.189 |
| R-free | 0.21014 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.650 | 2.450 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.061 | 0.431 |
| Number of reflections | 125199 | |
| Completeness [%] | 97.5 | 98.3 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | 20 %w/v PEG 3350 0.2 M (NH4)2SO4 0.1 M Na Acet pH=4.50 0.50 %w/v LDAO |
