5LVG

Hen Egg White Lysozyme co-crystallized with cis-Ru(DMSO)4Cl2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	120
Detector technology	CCD
Collection date	2015-11-26
Detector	AGILENT ATLAS CCD
Wavelength(s)	1.5418
Spacegroup name	P 43 21 2
Unit cell lengths	78.199, 78.199, 37.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.420 - 2.000
R-factor	0.1616
Rwork	0.158
R-free	0.23260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h87
RMSD bond length	0.016
RMSD bond angle	1.755
Data reduction software	CrysalisPro
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	14.520	14.520	2.050
High resolution limit [Å]	2.000	8.720	2.000
Rmerge	0.120	0.033	0.254
Number of reflections	8212
<I/σ(I)>	22.9
Completeness [%]	99.7	77.5	100
Redundancy	7.1	6.2	6.3
CC(1/2)	0.993	0.999	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	298	1M NaCl, 50 mM sodium acetate