5LU3
The Structure of Spirochaeta thermophila CBM64
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.703, 56.703, 66.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.210 - 1.500 |
| R-factor | 0.12506 |
| Rwork | 0.124 |
| R-free | 0.15107 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.522 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.210 | 1.530 |
| High resolution limit [Å] | 1.530 | 1.500 |
| Rmerge | 0.029 | 0.092 |
| Number of reflections | 18085 | |
| <I/σ(I)> | 79.4 | 16 |
| Completeness [%] | 99.9 | |
| Redundancy | 21.8 | 6.8 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 1% (w/v) Jeffamine ED-2001, 0.1M HEPES pH7.5 and 1M Succinic acid pH7.0 |
