5LTQ

Structure of the Yellow Fluorescent Protein lanYFP from Branchiostoma lanceolatum at pH 7.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	78.660, 197.160, 115.090
Unit cell angles	90.00, 90.45, 90.00

Refinement procedure

Resolution	49.290 - 2.050
R-factor	0.22677
Rwork	0.225
R-free	0.25371
Starting model (for MR)	pdbid 4JF9
RMSD bond length	0.006
RMSD bond angle	1.296
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.290		2.100
High resolution limit [Å]	2.050	7.000	2.050
Rmerge	0.076	0.022	0.585
Number of reflections	217268
<I/σ(I)>	13.54	46.21	2.4
Completeness [%]	99.4	98.5	99.6
Redundancy	3.8
CC(1/2)	0.998	0.999	0.718

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.19mM CYMAL-7, 40% PEG 400, 100mM HEPES