5LTP
Structure of the Yellow-Green Fluorescent Protein mNeonGreen from Branchiostoma lanceolatum at the acidic pH 4.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-03-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.870, 127.600, 146.840 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.340 - 1.700 |
R-factor | 0.18715 |
Rwork | 0.186 |
R-free | 0.21113 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jf9 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.499 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.340 | 1.800 | |
High resolution limit [Å] | 1.700 | 5.150 | 1.700 |
Rmerge | 0.042 | 0.016 | 0.582 |
Number of reflections | 291629 | ||
<I/σ(I)> | 13.78 | 49.34 | 1.58 |
Completeness [%] | 96.2 | 94.4 | 96 |
Redundancy | 4.3 | ||
CC(1/2) | 0.999 | 0.999 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 6.8mM CYMAL-7, 14% PEG 20,000, 100mM Sodium Citrate Tribasic Dihydrate |