5LTP

Structure of the Yellow-Green Fluorescent Protein mNeonGreen from Branchiostoma lanceolatum at the acidic pH 4.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-18
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	75.870, 127.600, 146.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.340 - 1.700
R-factor	0.18715
Rwork	0.186
R-free	0.21113
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jf9
RMSD bond length	0.008
RMSD bond angle	1.499
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.340		1.800
High resolution limit [Å]	1.700	5.150	1.700
Rmerge	0.042	0.016	0.582
Number of reflections	291629
<I/σ(I)>	13.78	49.34	1.58
Completeness [%]	96.2	94.4	96
Redundancy	4.3
CC(1/2)	0.999	0.999	0.632

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	6.8mM CYMAL-7, 14% PEG 20,000, 100mM Sodium Citrate Tribasic Dihydrate