5LTG

Citrate-bound Pichia angusta Atg18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 1
Unit cell lengths	57.811, 57.969, 62.173
Unit cell angles	84.19, 81.23, 87.01

Refinement procedure

Resolution	41.342 - 2.000
R-factor	0.2167
Rwork	0.214
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4av8
RMSD bond length	0.010
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.342	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.057	0.361
Number of reflections	51797
<I/σ(I)>	9.6
Completeness [%]	94.5
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	20 % (w/V) PEG 8000, 0.2 M NaCl, 0.1 M sodium phosphate dibasic-citric acid pH 4.2