5LTD
Phosphate-bound Pichia angusta Atg18
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.242, 58.253, 62.250 |
| Unit cell angles | 83.73, 80.85, 86.77 |
Refinement procedure
| Resolution | 45.000 - 2.500 |
| Rwork | 0.221 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ltg |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.133 | 0.645 |
| Number of reflections | 27376 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 98.3 | 96.5 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20 % (w/V) PEG 8000, 0.2 M NaCl, 0.1 M sodium phosphate dibasic-citric acid pH 4.2 |
